Cell Based Assays
Cell Based Assays
Invitrogen™ DAPI and Hoechst Nucleic Acid Stains
DAPI and Hoechst are classic popular nuclear counterstains for use in all fluorescent cell and tissue techniques. The blue fluorescence is vivid contrast to green, yellow, orange, red, far red and NIR fluorescent probes and labels.
Invitrogen™ eBioscience™ Propidium Iodide
Standard reagent used for assessing cell viability and exclusion of non-viable cells in flow cytometry. PI binds to double-stranded DNA, but is excluded from cells with intact plasma membranes.
Invitrogen™ CellVue™ Plum Cell Labeling Kit
Far-red fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ CellVue™ Jade Cell Labeling Kit
Green fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ eBioscience™ Propidium Iodide Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Propidium Iodide binds to double-stranded DNA by intercalating between basepairs, but is excluded from cells with intact plasma membranes.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 506/780 Sample Pack
Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.
Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.
Invitrogen™ eBioscience™ CFSE
Widely used for cell tracking and proliferation studies. It has also been used in CTL assays and cell motility studies. CFSE readily crosses intact cell membranes.
Invitrogen™ eBioscience™ Calcein Violet 450 AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Violet fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Violet 450.
Invitrogen™ Alexa Fluor™ 568 Phalloidin
High-affinity F-actin probe conjugated to Alexa Fluor 568 dye
Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 450
A violet fluorescent dye that can be used to monitor individual cell divisions for up to 7 generations.
Invitrogen™ Newport Green™ DCF Diacetate, cell permeant
Has moderate zinc-binding affinity (Kd for Zn2+ approximately 1μM) but is essentially insensitive to Ca2+
Invitrogen™ NP-EGTA (o-Nitrophenyl EGTA, Tetrapotassium Salt, cell impermeant
Photolabile chelator that exhibits high selectivity for Ca2+