Transfection Reagents
Transfection Reagents
MilliporeSigma™ Novagen™ ColiRollers™ Plating Beads
Used for plating bacteria for transformation molecular cloning applications.
Promega BL21(DE3)pLysS Competent Cells
BL21(DE3)pLysS Competent Cells allow high-efficiency protein expression of any gene that is under the control of a T7 promoter and has a ribosome binding site.
Promega ProFection™ Mammalian Transfection System
Calcium Phosphate-mediated transfection reagent used for transfection of DNA into many cell types.
Cell Biolabs Inc CytoSelect™ 96-Well Cell Transformation Assay Kit (Cell Recovery Compatible, Colorimetric), 96 assays
The traditional soft agar colony formation assay monitors anchorage-independent cell growth by measuring proliferation in a semisolid culture media in tissue culture dishes after 3-4 weeks, followed by manual counting of colonies. This method is cumbersome and not amenable to high throughput, and the manual counting of colonies is subjective because with varying colony sizes, it’s difficult to determine meaningful results. The CytoSelect™ 96-well Cell Transformation Assay (Cell Recovery Compatible) eliminates subjective manual counting of colonies with a much shorter assay time. Cells are incubated only 6-8 days in a semisolid agar media before being solubilized, lysed and detected by the provided colorimetric dye in a standard plate reader, making it a more quantitative and higher-throughput method. Additionally, the proprietary medium makes it possible to recover viable and intact cells for further downstream analysis. Each kit is sufficient for 96 tests in a microtiter plate.
New England Biolabs, Inc. NEB Turbo Competent E. coli (High Efficiency) – 20 x 0.05 ml/tube
Chemically competent E. coli competent cells suitable for high efficiency transformation and rapid colony growth.
Agilent Technologies SURE COMPETENT CELLS
SURE Competent Cells. For routine cloning of DNA with secondary structures, > /= 5 x 108 transformants/microgram. Restriction-Minus Competent Cells for Most Eukaryotic DNA. F- derivative for BAC cloning.
Agilent Technologies XL10-GOLD ULTRACOMP CELLS
XL10-Gold ultracompetent cells are created for transformation of large DNA molecules with high efficiency. Ideal for constructing plasmid DNA libraries, as they decrease size bias and produce larger, more complex plasmid libraries.
Agilent Technologies ELECTROTEN-BLUE E.COMP CELLS
ElectroTen-Blue Electroporation-Competent Cells 5 x 0.1mL and pUC18 control plasmid Highest electroporation efficiency (Hee) phenotype use for extrememly demanding cloning= 1 x 1010 transformants/microgram
Agilent Technologies XL1-BLUE SUB GRADE COMP CELLS
XL1-Blue Subcloning Grade Competent Cells. For cloning where DNA is not limited, >/=1 x 10^6 transformations/ug. Preparation of high-quality plasmid DNA. Allows blue-white color screening.
Agilent Technologies XL1BLUE MRF E.COMP CELLS
Cell, Electroporation-Competent, XL1-Blue MRF; Stratagene; For routine electroporation cloning applications; Ideal for cloning methyl; Restriction minus for cloning methylated DNA, greater than equal1 x 1010 transformants/ug
Agilent Technologies SURE ELECTROCOMP CELLS
SURE Electroporation-Competent Cells. For cloning DNA with secondary structures, > /= 1 x 1010 transformants/microgram. Clone unstable DNA, Increase stability of DNA containing inverted repeats or Z-DNA, Produce high-quality miniprep DNA
Agilent Technologies XL1-BLUE SUPERCOMPETENT CELLS
Cell, Supercompetent, XL1-Blue; Stratagene; For high-efficiency cloning, greater than equal1 x 109 transformant/ug; Preparation of quality plasmid DNA; Blue-white color screening; Contain antibiotic-resistant F episome; Versatile cloning
New England Biolabs, Inc. dam-/dcm- Competent E. coli – 6 x 0.2 ml/tube
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
Agilent Technologies XL1-BLUE MRF SUPERCOMP CELLS
XL1-Blue MRF' Supercompetent Cells. Restriction minus for cloning methylated DNA, > /= 1 x 109 transformants/ug
Agilent Technologies MBS MAMMAL TRANSFECTION KIT
Transfection MBS Mammalian Transfection Kit increases the efficiency of transfection by 100-fold or more, depending on the mammalian cell line to be transfected and also the yield of stable transformants