Immunoassay Reagents and Kits

Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.



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Invitrogen™ CD27 (Soluble) Human Instant ELISA™ Kit
Instant ELISA Kit


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ GM-CSF Human ELISA Kit
For the quantitative detection of human GM-CSF.


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Target Species | Human |
---|---|
Conjugate | HRP |
Interassay CV | 4.4% |
Intraassay CV | 6% |
Gene Symbol | GMCSF |
Total Assay Time | 3 hr. 10 min. |
Sample Volume | Amniotic Fluid, 50 μL; Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | 2.9 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 7.8-500 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 20 min. |
Accession Number | P04141 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 1437 |
Sample Type | Amniotic Fluid,Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Molgramostin, Sargramostim |
Invitrogen™ IL-2 Mouse ELISA Kit
For the quantitative detection of mouse IL-2.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Target Species | Mouse |
---|---|
Conjugate | Biotin |
Interassay CV | 10% |
Intraassay CV | 5% |
Gene Symbol | IL-2 |
Total Assay Time | 3 hr. 10 min. |
Sample Volume | Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | 5.3 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 15.6-1,000 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 20 min. |
Accession Number | P04351 |
Regulatory Status | RUO |
Target | Mouse |
Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 16183 |
Sample Type | Serum,Supernatant |
Label or Dye | HRP |
Synonym | Aldesleukin, T cell growth factor, TCGF |
Invitrogen™ IL-6 Rat ELISA Kit
For the quantitative detection of rat IL-6.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Target Species | Rat |
---|---|
Conjugate | Biotin |
Interassay CV | 10% |
Intraassay CV | 5% |
Gene Symbol | ILg6,Ifnb2 |
Total Assay Time | 3 hr. 10 min. |
Sample Volume | Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | 12.0 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 31.3-2,000 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 20 min. |
Accession Number | P20607 |
Regulatory Status | RUO |
Target | Rat |
Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 24498 |
Sample Type | Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interferon-beta 2, B-cell stimulatory factor 2,HGF |
Invitrogen™ IL-17A Rat ELISA Kit
For the quantitative detection of rat IL-17A.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Target Species | Rat |
---|---|
Conjugate | Biotin |
Interassay CV | 7.6% |
Intraassay CV | 8.5% |
Gene Symbol | CTLA-8,IL-17,IL-17A,Il17 |
Total Assay Time | 3 hr. 30 min. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | 1.0 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 1.6-100 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 20 min. |
Accession Number | Q61453 |
Regulatory Status | RUO |
Target | Rat |
Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 301289 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin 17 A, CTLA8 |
Invitrogen™ IL-4 Mouse Uncoated ELISA Kit with Plates
Specifically engineered for accurate and precise measurement of mouse IL-4 protein levels from samples including serum, and cell culture supernatants.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Target Species | Mouse |
---|---|
Conjugate | Biotin |
Gene Symbol | IL-23,p19,Il-12b,Il-12p40,Il12p40,p40 |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 4 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 4-500 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P07750 |
Regulatory Status | RUO |
Target | Mouse |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 16189 |
Sample Type | Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin-4 il4 |
Invitrogen™ IL-1 beta Human ELISA Kit
For the quantitative detection of human IL-1beta.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 8.6% |
Intraassay CV | 5.1% |
Gene Symbol | IL-1,IL1-BETA,IL1F2 |
Total Assay Time | 3 hr. 10 min. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Urine,50 μL |
Assay Sensitivity | 0.3 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 3.9-250 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 20 min. |
Accession Number | P01584 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Adhesive Plate Covers,Standard,Sample Diluent,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Wash Buffer,Controls,Chromogen,Stop Solution |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3553 |
Sample Type | Plasma,Serum,Supernatant,Urine |
Label or Dye | HRP |
Synonym | Catabolin, Interleukin-1 beta, IL1F2 |
Invitrogen™ TNF alpha Human ELISA Kit
For the quantitative detection of human TNF-alpha.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 7.4% |
Intraassay CV | 6% |
Gene Symbol | DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF |
Total Assay Time | 3 hr. 10 min. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Urine,50 μL |
Assay Sensitivity | 2.3 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 7.8-500 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 20 min. |
Accession Number | P01375 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Assay Buffer concentrate,SAV-HRP,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Stop Solution |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 7124 |
Sample Type | Plasma,Serum,Supernatant,Urine |
Label or Dye | HRP |
Synonym | Cachectin, Tumor necrosis factor alpha, DIF |
Invitrogen™ TNF alpha Human Instant ELISA™ Kit
Instant ELISA Kit


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ pNPP Powder
Suitable for use in immunoassays


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Invitrogen™ IFN gamma Human Uncoated ELISA Kit
Uncoated ELISA Kit


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Target Species | Human |
---|---|
Conjugate | Biotin |
Gene Symbol | IL-12p35,Il-12a,Ll12a,p35,Il-12b,Il-12p40,Il12p40,p40 |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 4 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 4-500 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P01579 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3458 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interferon-gamma, IFN-g, IFNg |
Invitrogen™ IL-12 p70 Human Uncoated ELISA Kit
Uncoated ELISA Kit


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Target Species | Human |
---|---|
Conjugate | Biotin |
Gene Symbol | IFN-g,Ifg |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 4 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 4-500 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P29459,P29460 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Streptavidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3592, 3593 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin-12, IL12, IL23 |
Invitrogen™ APRIL Human ELISA Kit
For the quantitative detection of human APRIL.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 7.1% |
Intraassay CV | 8.1% |
Gene Symbol | APRIL,CD256,TALL-2,TALL2,TNLG7B,TRDL-1,UNQ383/PRO715,ZTNF2 |
Total Assay Time | 3 hr. 10 min. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | 0.4 ng/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 0.78-50.0 ng/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 20 min. |
Accession Number | O75888 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Adhesive Plate Covers,Sample Diluent,Sample Diluent,Biotinylated Detection Antibody,SAV-HRP,SAV-HRP,Lysis Buffer,Chromogen,Chromogen,Stop Solution |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 8741 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | TNF 13, TALL 2, TRDL 1, CD256 antigen |
Invitrogen™ Myeloperoxidase Human Instant ELISA™ Kit
Instant ELISA Kit


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ IL-10 Human Uncoated ELISA Kit
Uncoated ELISA Kit


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Target Species | Human |
---|---|
Conjugate | Biotin |
Gene Symbol | BCGF-1,BCGF1,BSF-1,BSF1,IL-4 |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 2 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 2-300 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P22301 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3586 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin-10 |